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Markus Sperandio, MD
Walter-Brendel-Center of Experimental Medicine
Ludwig-Maximilians-Universität
Marchioninistr. 27
81377 München
Germany
+49 89-2180 76505
+49 89-2180 76503
Email: markus.sperandio@med.uni-muenchen.de
Website: http://www.wbex.med.uni-muenchen.de/
Our lab investigates the molecular mechanisms of leukocyte recruitment using intravital microscopy and several static and dynamic in vitro assays. Leukocyte recruitment proceeds along a well-defined cascade of events starting with selectin-dependent leukocyte capture to and rolling along the vessel wall. During rolling, leukocytes get into intimate contact with the endothelium, which allows endothelial-bound chemokines to interact with specific chemokine-receptors on leukocytes. This in turn triggers the activation of integrins leading to firm arrest and transmigration into tissue. We are interested in all aspects of leukocyte recruitment during inflammation in vivo. This includes the role of posttranslational glycosylation on selectin ligand, chemokine-receptor function and integrin activation. Recently, we have begun to work on a novel intravital microscopy model in the mouse embryo, which provides us with a powerful new tool to investigate the maturing immune system during development in different embryonic and extraembryonic compartments.
Group members
Monika Prünster, PhD
Stephan Schmid
Natalie Schweiger
Anna Gloning
Susanne Bierschenk
Recent publications
Frommhold D, Ludwig A, Bixel MG, Zarbock A, Babushkina I, Weissinger M, Lange-Sperandio B, Ellies LG, Marth JD, Beck-sickinger AG, Zernecke A, Weber C, Vestweber D, Ley K, Sperandio M. Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during inflammation . J Exp Med 2008;205(6):1435-46.
Yakubenia S, Frommhold D, Schölch D, Hellbusch CC, Korner C, Petri B, Jones C, Ipe U, Bixel G, Krempien R, Sperandio M, Wild MK. Leukocyte trafficking in a mouse model for Leukocyte Adhesion Deficiency II / Congenital Disorder of Glycosylation IIc. Blood 2008;112(4):1472-81.
Zernecke A, Bot I, Talab YD, Shagdarsuren E, Bidzhekov K, Meiler S, Krohn R, Schober A, Sperandio M, Soehnlein O, Bornemann J, Tacke F, Biessen EA, Weber C. Protective Role of CXC Receptor 4/CXC Ligand 12 Unveils the Importance of Neutrophils in Atherosclerosis. Circ Res 2008;102(2):209-17.
Frommhold D, Mannigel I, Schymeinsky J, Mocsai A, Poeschl J, Walzog B, Sperandio M. Syk is critical for sustained leukocyte adhesion during inflammation in vivo. BMC Immunol 2007;8(31):1-8.
Hellbusch CC, Sperandio M, Frommhold D, Yakubenia S, Wild MK, Popovici D, Vestweber D, Grone HJ, von Figura K, Lubke T, Korner C. Golgi GDP-fucose transporter-deficient mice mimic congenital disorder of glycosylation IIc/Leukocyte adhesion deficiency II. J Biol Chem 2007 February 2;282(14):10762-72.
Schymeinsky J, Sindrilaru A, Frommhold D, Sperandio M, Gerstl R, Then C, Mocsai A, Scharffetter-Kochanek K, Walzog B. The Vav binding site of the non-receptor tyrosine kinase Syk at Tyr 348 is critical for beta2 integrin (CD11/CD18)-mediated neutrophil migration. Blood 2006 August 1;108(12):3919-27.
Sperandio M, Pickard J, Unnikrishnan S, Acton ST, Ley K. Analysis of leukocyte rolling in vivo and in vitro. Methods Enzymol 2006;416:346-71.
Sperandio M. Selectins and glycosyltransferases in leukocyte rolling in vivo. FEBS J 2006 October;273(19):4377-89.
Sperandio M, Frommhold D, Babushkina I, Ellies LG, Olson TS, Smith ML, Fritzsching B, Pauly E, Smith DF, Nobiling R, Linderkamp O, Marth JD, Ley K. alpha2,3-Sialyltransferase-IV is essential for L-selectin ligand function in inflammation. Eur J Immunol 2006 December;36(12):3207-15.
Smith ML, Sperandio M, Galkina EV, Ley K. Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P-selectin and E-selectin. J Leukoc Biol 2004 November;76(5):985-93.
Sperandio M, Smith M.L., Forlow SB, Olson TS, Xia L, McEver RP, Ley K. P-selectin Glycoprotein Ligand-1 mediates L-selectin-dependent leukocyte rolling in venules. J Exp Med 2003;197(10):1355-63.