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Markus Sperandio, MD
Walter-Brendel-Center of Experimental Medicine
Ludwig-Maximilians-Universität
Marchioninistr. 27
81377 München
Germany
+49 89-2180 76505
+49 89-2180 76503
Email: markus.sperandio@med.uni-muenchen.de
Website: http://www.wbex.med.uni-muenchen.de/
Our lab investigates the molecular mechanisms of leukocyte recruitment using intravital microscopy and several static and dynamic in vitro assays. Leukocyte recruitment proceeds along a well-defined cascade of events starting with selectin-dependent leukocyte capture to and rolling along the vessel wall. During rolling, leukocytes get into intimate contact with the endothelium, which allows endothelial-bound chemokines to interact with specific chemokine-receptors on leukocytes. This in turn triggers the activation of integrins leading to firm arrest and transmigration into tissue.
We are interested in all aspects of leukocyte recruitment during inflammation in vivo. This includes the role of posttranslational glycosylation on selectin ligand, chemokine-receptor function and integrin activation. Recently, we have begun to work on a novel intravital microscopy model in the mouse embryo, which provides us with a powerful new tool to investigate the maturing immune system during development in different embryonic and extraembryonic compartments.
Group members
Postdocs:
Claudia Nussbaum, MD
Monika Prünster, PhD
MD students:
Stephan Schmid
Natalie Schweiger
Anna Gloning
Janine Abisch
Technician:
Susanne Bierschenk
Recent selected publications
D. Frommhold, A. Kamphues, I. Hepper, M. Pruenster, I.K. Lukić, I. Socher, V. Zablotskaya, K. Buschmann, B. Lange-Sperandio, J. Schymeinsky, E. Ryschich, J. Poeschl, C. Kupatt, P.P. Nawroth, M. Moser, B. Walzog, A. Bierhaus, and M. Sperandio. RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo. Blood, First Edition 2010.
V. Marcos, C. Nussbaum, L. Vitkov, A. Hector, E. M. Wiedenbauer, D. Roos, T. Kuijpers, W. D. Krautgartner, O. Genzel-Boroviczény, M. Sperandio, and D. Hartl. Delayed but functional neutrophil extracellular trap formation in neonates. Blood (Letter) 2009;114: 4908-4911.
M. Sperandio, C. Gleissner, and K. Ley. Glycans in inflammation and lymphocyte trafficking. (Invited review). Immunol. Rev. 2009; 230: 97-113.
J. Schymeinsky, R. Gerstl, I. Mannigel, K. Niedung, D. Frommhold, K. Panthel, J. Heesemann, M. Sixt, T. Quast, W. Kolanus, A. Mocsai, J. Wienands, M. Sperandio, and B. Walzog. A fundamental role of mAbp1 in neutrophils: impact on β2 integrin-mediated phagocytosis and adhesion in vivo. Blood 2009; 114:4209-20.
E. Ryschich, V. Kerkadze, O. Deduchovas, O. Salnikova, A. Parseliunas, A. Märten, W. Hartwig, M. Sperandio, J. Schmidt. Intracapillary leukocyte accumulation as a novel antihemorrhagic mechanism in acute pancreatitis in mice. Gut 2009; 58:1508-16.
Z. Jakus, E. Simon, D. Frommhold, M. Sperandio and A. Mócsai. Critical Role of Phospholipase C in Integrin- and Fc-Receptor-Mediated Neutrophil Functions and the Effector Phase of Autoimmune Arthritis. J.Exp.Med. 2009; 206: 577-93.
M. Moser, M. Bauer, S. Schmid, R. Ruppert, S. Schmidt, M. Sixt, H. Wang, M. Sperandio, R. Fässler. Kindlin-3 is required for β2 integrin-mediated leukocyte adhesion to endothelial cells. Nat.Med. 2009; 15: 300-305.
S. Yakubenia, D. Frommhold, D. Schölch, C. Hellbusch, C. Körner, C. Jones, B. Petri, U. Ipe, M.G. Bixel, R. Krempien, M. Sperandio*, and M.K. Wild*. Leukocyte trafficking in a mouse model for Leukocyte Adhesion Deficiency II / Congenital Disorder of Glycosylation IIc. Blood 2008; 112:1472-81. (*equal contribution)
D. Frommhold, A. Ludwig, M.G. Bixel, A. Zarbock, I. Babushkina, M. Weissinger, B. Lange-Sperandio, L.G. Ellies, J.D. Marth, A.G. Beck-Sickinger, A. Zernecke, C. Weber, D. Vestweber, K. Ley, and M. Sperandio. Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during inflammation. J. Exp. Med. 2008; 205(6):1435-46.
A. Zernecke, I. Bot, Y.D. Talab, E. Shagdarsuren, F. Tacke, S. Meiler, E.A. Liehn, A. Schober, M. Sperandio, E.A. Biessen, C. Weber. The protective role of CXCR4/CXCL12 unveils an importance of neutrophil homeostasis in atherosclerosis. Circ.Res. 2008; 102(2):209-17.
D. Frommhold, I. Mannigel, J. Schymeinsky, A. Mocsai, J. Poeschl, B. Walzog, and M. Sperandio. Syk is critical for sustained leukocyte adhesion during inflammation in vivo. BMC Immunol. 2007; 8(31) 1-8.
C. Hellbusch, M. Sperandio, D. Frommhold, M. Wild, D. Vestweber, H.J. Gröne, K. von Figura, T. Lübke and C. Körner. Defective selectin binding, abnormal lung morphology and evidence for an alternative GDP-fucose transport mechanism in Golgi GDP-fucose transporter deficient mice. J.Biol.Chem. 2007; 282:10762-72.
J. Schymeinsky, A. Sindrilaru, D. Frommhold, M. Sperandio, R. Gerstl, C. Then, K. Scharffetter-Kochanek, and B. Walzog. The Vav binding site of the non-receptor tyrosine kinase Syk at Tyr 348 is critical for β2 integrin (CD11/CD18)-mediated leukocyte polarization and migration. Blood 2006; 108: 3919-3927.
M. Sperandio. Selectins and glycosyltransferases in leukocyte rolling. (Invited review for Minireview Series). FEBS J. 2006; 273:4377-89.